lung altered expression of il-1β mrna and its signaling pathway molecules in obese-asthmatic male wistar rats
نویسندگان
چکیده
epidemiological and clinical studies indicate a close relationship between obesity and asthma. here, we determined the impact of diet-induced obesity on the expression levels of il-1β, irak-1 and traf-6 mrna as well as il-1β protein level and pathological changes in male wistar rat’s lung after sensitization with ovalbumin (ova). twenty male wistar rats divided into four groups, control with normal diet (c+nd), ova-sensitized with normal diet (s+nd), control with high-fat diet (c+hfd), and ova-sensitized with high-fat diet (s+hfd). all rats fed for 12 weeks with standard pellets or high-fat diet while sensitization and challenging with ova or saline were done for groups in the last month. in the end of intervention, lung was isolated and tested for the expression levels of il-1β, irak-1 and traf-6 mrna with real time-pcr method, and pathological changes were determined. diet-induced obesity groups showed increased weight, obesity indexes and lipid profiles the expression levels of il-1β mrna in ova-sensitization groups (s+nd and s+hfd) showed a significant increase compared with other groups. also in s+hfd group, expression level of irak-1 and traf-6 mrna were markedly higher than other groups ( p <0.001). the pathological changes were marked in sensitized groups compared to non-sensitized groups; with marked increase in obese sensitized rat. the results showed that high fat diet caused overexpression of il-1β, irak-1 and traf-6 mrna as well as il-1β protein in an experimental model of asthma. our results suggest that obese-asthmatic conditions may lead to the local production and activation of pro-inflammatory agents.
منابع مشابه
Tracheal overexpression of IL-1β, IRAK-1 and TRAF-6 mRNA in obese-asthmatic male Wistar rats
Objective(s): Human and animal studies have shown a close relationship between obesity and asthma severity. Here, we examined the effects of diet-induced obesity (DIO) on the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA in male Wistar rats tracheal after sensitization with ovalbumin (OVA). Materials and Methods:Twenty male Wistar rats divided to four groups, included, control group with ...
متن کاملTracheal overexpression of IL-1β, IRAK-1 and TRAF-6 mRNA in obese-asthmatic male Wistar rats
OBJECTIVES Human and animal studies have shown a close relationship between obesity and asthma severity. Here, we examined the effects of diet-induced obesity (DIO) on the expression levels of IL-1β, IRAK-1 and TRAF-6 mRNA in male Wistar rats tracheal after sensitization with ovalbumin (OVA). MATERIALS AND METHODS Twenty male Wistar rats divided to four groups, included, control group with no...
متن کاملtracheal overexpression of il-1β, irak-1 and traf-6 mrna in obese-asthmatic male wistar rats
objective(s): human and animal studies have shown a close relationship between obesity and asthma severity. here, we examined the effects of diet-induced obesity (dio) on the expression levels of il-1β, irak-1 and traf-6 mrna in male wistar rats tracheal after sensitization with ovalbumin (ova). materials and methods:twenty male wistar rats divided to four groups, included, control group with n...
متن کاملتاثیر تمرین هوازی پس از استنشاق ریزذرات کربن سیاه بر بیان IL-β بافت ریه موش‌های صحرایی نر: گزارش کوتاه
Background: Air pollution PM exposure associated with increased inflammation and decreased cellular immune function. The innate immune system is one of the first lines of defense against inhaled air pollution and is characterized by activation of lung key signaling pathways. One pathway is initiated by the activation of toll-like receptors (TLRs) that associated with airway cells culminates in ...
متن کاملNeuroprotective Effects of Berberine After Severe Traumatic Brain Injury in Male Rats: The Role of IL-1β and IL 10
Background and purpose: Traumatic brain injury (TBI) is the leading cause of death in young people. Berberine is a flavonoid rich in barberries and many traditional Iranian herbal remedies that could be used in treatment of neurodegenerative diseases. These properties make it a viable treatment for neurodegenerative diseases. Therefore, this study intended to investigate the neuroprotective ac...
متن کاملthe effect of aqueous extract of garlic on formalin-induced pain in male rats
چکیده اثر عصاره آبی سیر بر درد ناشی از آزمون فرمالین در موش صحرایی نر به کوشش نرگس اسکندری روزبهانی زمینه و هدف: گیاه سیر از خانواده لیلیاسه و گونهallium sativum بومی آسیای میانه بوده و از دوران ،باستان تاکنون به خواص درمانی متفاوت آن مثل: کاهندگی قندخون، کلسترول خون، فشار خون، اثرات مفیدآن بر دستگاه قلبی عروقی و بیماریهای انعقادی خون، اثرات آنتی اکسیداتیو، درمان بیماریهای تنفسی وگوارشی، ا...
15 صفحه اولمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of allergy, asthma and immunologyجلد ۱۵، شماره ۳، صفحات ۱۸۳-۱۹۷
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023